Sentence examples for mouse megakaryocytes compared from inspiring English sources
We observed that CIB1 expression is increased in primary mouse megakaryocytes compared to mononuclear bone marrow cells as determined by Western blot analysis.
The red pulp compartment showed a significantly (P < 0.05) increased average number of megakaryocytes compared with uninfected mice, which may be associated with hematopoietic reconstitution.
Instead several of these ncRNAs and their isoforms are upregulated in megakaryocytes compared to HSCs (Supplementary Fig. 6c, d; validated by qRT-PCR in Fig. 5c, d) and are retained in thrombocytes (Fig. 5c).
In agreement with our Dami cell data, we noticed that Cib1 protein expression was elevated in the megakaryocytes compared to the mononuclear cells (figure 1C).
Similar analyses of tetraspanins, however, revealed that none were specific to megakaryocytes (results not shown), although the fact that nine of the tetraspanins had only five tags or less made it difficult to determine statistically whether any of these were over-represented in megakaryocytes compared with other cell types.
The scientists first compared mouse megakaryocyte cells created from embryonic stem cells engineered to lack p45-Nfe2 with normal megakaryocytes.
To conclusively determine if the increase in CIB1 protein expression observed in Dami cells treated with PMA has physiological merit, we analyzed Cib1 expression in mouse mononuclear bone marrow cells as well as mouse megakaryocytes.
SAGE identified 16 tetraspanins in primary mouse megakaryocytes.
Primary mouse megakaryocytes were cultured in a serum-free system as described previously.
Indeed, expression of Tspan9 protein in primary mouse megakaryocytes, and the proplatelet structures that they form, was confirmed by fluorescence microscopy, a technique that was used because of difficulties in obtaining enough mature megakaryocytes for Western blotting.
Fetal liver mouse megakaryocytes were prepared and cultured as described [ 20], and mature megakaryocytes were collected after five days by passing the culture over a bovine serum albumin step gradient.
