For T98G cells the plating efficiencies were 0.48 ± 0.07 for cells receiving serum from unirradiated mice and 0.48 ± 0.06 for cells receiving serum from irradiated mice. 2 μg/ml TGF-β3 neutralizer (AF243-NA, R&D systems, Minneapolis, MN, USA) was added to the medium containing mouse serum before transfer to reporter cells.
Neurospheres were plated onto poly-d-lysine coated coverslips in NeuroCult™ NSC basal medium containing mouse NeuroCult™ NSC proliferation supplements and 2% fetal calf serum (Sigma-Aldrich) without growth factors.
TGF-β3 neutralizer added to the medium containing mouse serum inhibited the effect.
Bone marrow-derived macrophages were cultured from mouse bone marrow in medium containing 20 ngml − recombinant macrophage colony-stimulating factor (see the Supplemental Data).
Forty-eight hours after transfection, the cells were incubated in cold DMEM medium containing mouse M1 anti-FLAG antibody at 2 µg·mL–1 and incubated 1 h at 4°C.
Culturing mouse testicular cells in medium containing GDNF and/or LIF does not alter methylation of the paternal imprinted H19 locus, indicating that growth factors do not alter DNA methylation per se [ 66].
The human embryonic stem cell line, H1 (WiCell, Madison, WI, USA), was grown on irradiated mouse embryonic fibroblasts in medium containing Dulbecco's modified Eagle medium (DMEM):F12, serum replacer (Invitrogen, Paisley, UK), l-glutamine, β-mercaptoethanol, non-essential amino acids and basic fibroblast growth factor (8 ng/ml).
To further investigate the significance of pancIl17d for embryonic development, we plated the surviving pancIl17d knockdown blastocysts in medium containing mouse LIF and inhibitors for MEK1/2 (MandK1/2) and GSK3β (2i medium), conditions that are frequently utilized for the culture of ground-state ESCs, and harvested the cultures after 10 days.
Finally, hyperplastic ear skin from the transgenic mice was incubated in medium containing mMCP-4 and subsequently embedded in a collagen gel containing randomly dispersed bovine capillary endothelial cells (BCEs).
To test whether the tumour cells retained their dependence on oestrogen signalling in vitro, cells explanted from primary tumours in mice were plated in medium containing either oestradiol or fulvestrant.
