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In addition, eugenol suppressed the cyclooxygenase-2 (COX-2) gene expression in LPS-stimulated mouse macrophage cells.
Our observations are consistent with previous studies in human alveolar epithelial cells and mouse macrophage cells which reported that PEGylated NPs formed smaller intracellular agglomerates than did bare AuNPs [22, 39] and experienced decreased cellular uptake [40].
We designed to investigate the inhibitory effect of hesperidin on lipopolysaccharide (LPS -induced over-expression of cycLPS -induced2 (Cover-expressione nitric ofide synthase (iNOS) proteins, over-production of prostaglandin E2 (PGE2) and nitric oxide (NO) using mouse macrophage cells.
In this focused pipeline, the targets for structure determination are proteins that are expressed in mouse macrophage cells and that are inferred to have a role in innate immunity.
A co-culture model of RAW 264.7 mouse macrophage cells and HT-29 human intestinal epithelial cells was established to study the correlation between inflammation and aquaporin (AQP) expression and to evaluate the toxicity of different diterpenoids from E. pekinensis.
The most active peptide was further evaluated in vitro for RANKL induced osteoclastogenesis in mouse macrophage cells, and in vivo for Freund's complete adjuvant induced arthritis (AIA) in Lewis rats.
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The RAW264.7 mouse macrophage cell line was obtained from ATCC.
aureus infected mouse macrophage cell line RAW264.7 (P < 0.01).
The mouse macrophage cell line, RAW 264.7, was obtained from the American Type Culture Collection.
The mouse macrophage cell line RAW264.7 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Ana-1 cell line (a mouse macrophage cell line) was purchased from Shanghai Cell Bank, the Chinese Academy of Sciences (Shanghai, China).
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