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If rabbits receive repeated injections of mouse lymphocytes, they become immunized and develop antibodies against the mouse cells.
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A single-cell DNA electrophoresis (DNA comet assay) was employed in order to assess NPs cytotoxicity towards mouse lymphocytes in vivo.
AgcoreAushell NPs showed low genotoxicity towards mouse lymphocytes as evidenced by DNA comet analysis and did not affect hepatic lipid and protein oxidation.
Additionally, compound 300 showed moderate inhibition of non-specific esterase activity in mouse lymphocytes up to 44.2% compared with control cells at concentration 100 µg/mL.
Transduction of GT-KO mouse lymphocytes with replication defective adenovirus containing the α1,3GT gene (AdαGT) results in presentation of α-gal epitopes on these lymphocytes.
AgcoreAushell NPs upon their administration to mice with LLC possessed low in vivo genotoxicity towards untransformed cells as evidenced by single-cell DNA comet analysis of primary mouse lymphocytes (Fig. 7).
20 μL of blood (treated with Heparin sodium) from each mouse was collected for flow cytometry test respectively at Day 7, 14 or 21 after treatment, the percentage ratios of human CD3+ T cells to mouse lymphocytes in peripheral blood were showed.
We also noticed low levels of galectin-3 binding to mouse lymphocytes.
Synthesis of catecholamines in mouse lymphocytes, and their increased levels in the activated state was noticed earlier [63], [65].
We next evaluated IL-2 secretion from CD3/CD28 expanded primary mouse lymphocytes by TCR stimulation with anti-CD3 antibody coated on 96-well plates (Fig. 8A).
After 2 3 weeks, CB-SC growing at 80 90% confluence were co-cultured with mouse lymphocytes after removing all unattached cord blood mononuclear cells.
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