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Both viruses were able to replicate in mouse lungs; however, the H9N2 virus grew to significant higher titers than the H7N9 virus.
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It has to be pointed out that the human cell population underwent ex vivo expansion before implantation of c-kit-positive clones into an injured mouse lung; however, similar results have been described with non-clonal cells.
In adult mice lungs, however, autophagy is increased upon mechanical ventilation and contributes to cellular damage [41].
All six mycoplasma-free cell lines engrafted in scid mice metastasised to the lungs; however, considerable variation concerning their growth in scid mice, their metastatic potential and glycoconjugate expression was observed (as summarised in Table 1; cell lines are listed according to their metastatic potential from low to high).
Adams has a photograph of those mouse lungs above his desk.
WT mouse lungs remained negative.
Of note however, mouse lung structure is not identical to the lung structure in humans.
However, one mouse lung had three small metastatic nodules (indicated by red arrows in Figure 5), which differed slightly from the bioluminescence imaging result in Figure 4A.
The role of human tumor versus mouse lung-derived cytokines directing this tropism, however, cannot be ruled out.
However, the majority of mouse lung tumors are adenomas [33], [37], [38], which are thought to be adenocarcinoma precursors.
Only the mouse lung epithelial cell line MLE-12 showed any significant toxicity, however all of the cell lines were able to internalize the particles.
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