Sentence examples for mouse lung using from inspiring English sources
Williams et al performed miRNA profiling at 3 time points (P1, P14 and P60) of the developing mouse lung using a panel of 156 individual miRNAs [17].
Mass spectral data sets were acquired over each whole mouse lung using flexImaging™ software (Bruker Daltonik, Bremen, Germany) in the mass range of m/z 500 1200 with a raster step size of 100 µm and 250 laser shots per spectrum.
Briefly, total RNA was isolated from E18.5 mouse lung using TriReagent (Sigma) and 10 μg was used for 5' RLM-RACE, to facilitate the cloning of the full-length Fgf-10 5' cDNA sequence.
Bicistronic lentiviruses expressing Sox2 and Cre recombinase are delivered specifically to the mouse lung using intranasal inhalation, allowing constitutive expression of two genes driven by β -Actin and Pgk promoters, respectively.
Total RNA was extracted from the excised apical lobe of the right mouse lung using an RNeasy mini kit (Qiagen) and reverse transcribed using 5 μg of random hexamers (Omniscript RT kit, Qiagen) as primers.
In order to explore the role of K14 expression in pulmonary epithelium that normally lacks both squamous differentiation and K14 expression, human keratin 14 gene hK14 was constitutively expressed in mouse lung using a Clara cell specific 10 kDa protein (CC10) promoter.
Influenza A mCherry viruses were also able to lethally infect mice, and strikingly, dose- and time-dependent kinetics of viral replication were monitored in whole excised mouse lungs using an in vivo imaging system (IVIS).
Total lung RNA was extracted from mouse lungs using TRIzol reagent (Invitrogen Life Technologies, USA and reversed transcribed into cDNA (Advantage RT-for-PCR Kit; Clontech, USA).
To measure lung micrometastasis formation, we quantified the portion of human DNA in mouse lungs using real-time PCR for human Alu sequences, as previously described (Schneider et al, 2002).
To determine potential impact in global DNA methylation patterns in the lungs of the transgenic mice elicited by ΔDNMT3B4- del overexpression, we first analyzed the overall levels of DNA methylation in the genome of the mouse lungs using Imprint® Methylated DNA Quantification kit (Sigma).
We identified significant miRNA-mediated biological process categories in developing mouse cerebellum and lung using non-targeted mRNA expression as the negative control.
