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The VDRGS was similarly found to overlap significantly with the DLCGS of an independent developing mouse lung time series (E12 to P30), GSE20954 (OR 2.07, 95% CI: 1.60-2.68) [ 20].
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Masson trichrome staining for collagen and α-SMA immunostaining indicated severe tissue fibrosis of mouse lung at later time points (Zhang et al., 1996) (Fig. S5A C).
This is consistent with findings showing marked up-regulation of the HSPs in mouse lung at early times after naphthalene treatment (Williams et al. 2003).
Recent work by our group demonstrated that 117 significant miRNAs were dynamically regulated during mouse lung organogenesis from 7 time points compassing all recognized stages of lung development beginning at embryonic day 12 and continuing to adulthood [ 11].
Cd exposure significantly increased MT in homogenates of total lung in both species; however, no significant increase of MT in rat lung tissue after removal of free lung cells by lavage was found whereas MT was still significantly increased in lavaged mouse lung tissue throughout the postexposure time.
Figure 4 displays the result obtained by two-way clustering genes along 7 time points of the mouse lung development.
To measure lung micrometastasis formation, we quantified the portion of human DNA in mouse lungs using real-time PCR for human Alu sequences, as previously described (Schneider et al, 2002).
Williams et al performed miRNA profiling at 3 time points (P1, P14 and P60) of the developing mouse lung using a panel of 156 individual miRNAs [17].
In mice, we assessed gene expression profiles in mouse lung, brain and PBMC associated with a lung inflammation during a 24-hour time course.
To quantify Mp rapidly from infected tissues, we developed a real-time PCR-based method for measuring the numbers of Mp in extracts of mouse lung.
Using transgenic mice in a pre-clinical model and detailed in vitro studies, we not only establish a causal relationship between the two pathways through the lung MFB but, at the same time, show that PDGF treatment is able to successfully restore VEGF signaling in the newborn mouse lung ([50] (in press).
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