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Our goal was to characterize basic features of HPV in the isolated buffer-perfused and ventilated mouse lung system.
All 3 pathways could be causally connected to carcinogenesis both in this mouse lung system and in other species and target organs.
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Expanded human SOX9+ BCs can give rise to alveolar and bronchiolar epithelium after being transplanted into injured mouse lung, with air-blood exchange system reconstructed and recipient's lung function improved.
Benz(a)anthracene, 7,12-dimethylbenz(a)anthracene, dibenz(a)anthracene and benzo(a pyrene and their related "K region" epoxides were tested for carcinogenic activities using a system in which mouse lung tissue was incubated in the presence of the test compound for 30 min and then implanted into isologous mice.
Here, to facilitate comparisons, we used the same tet-inducible system [9] to develop mouse lung tumor models that express the EGFRT790M mutant.
Using magnetic Fe3O4 nanoparticles as reporters, it has been demonstrated that approximately 0.4 µg of nanoaerosol was deposited into a mouse lung in 1 h in a nose-only exposure system.
Here, we describe a system to introduce exogenous antigens into genetically engineered mouse lung cancers to mimic tumor neoantigens.
Mice were immunized intraperitoneally with 100 μl of Sigma Adjuvant (Sigma-Aldrich, St . Louis USA) alone or in combination with 10 μg mouse lung collagen (Elastin Products, Owensville, USA), 10 μg recombinant mouse decorin (R&D Systems, Minneapolis, USA) and 10 μg mouse lung elastin peptides (Elastin Products).
It was hypothesized that CO3Ap is an efficient gene delivery system to the lung cell lines and can be subsequently used for gene transfer to the mouse lung.
Gene expression from three replicates of three different RNA sources (mouse whole lung, mouse lung cell line, and Stratagene Universal mouse reference RNA) were evaluated with six different technologies encompassing different reporter systems (short oligonucleotides, long oligonucleotides, and cDNAs), labeling techniques, and hybridization protocols.
In the mouse lung, ILC2s are defined as Lin−CD90+ICOS+CD25+ST2+CD127+.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com