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On the other side, after intratracheally aspiration of AuNPs, some GFP+ part of mouse lung showed significant gold signal, indicating the regenerated human tissues are anatomically linked with atmospheric air (Figs. 4G and S7C).
While H&E staining revealed the occasional presence of macrophages in untreated Npc1−/− lung, CD-treated Npc1−/− mouse lung showed more macrophages present and also increasing macrophage infiltration the longer CD-treatment was continued (Fig. S3C).
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Additional file 3: Changes in E14.5 Apc CKO mouse lung, shown by a series of H&E-stained horizontal tissue sections.
From combined flow cytometry and gene expression analysis of isolated cell subpopulations from infected mouse lungs showed that neutrophil influx was largely responsible for the predictive transcriptional signatures.
Despite the presumed virulence of the strain--experiments with mouse lungs showed it produces 1000 times more bacteria in infected cells than do standard varieties--Valway says the number of TB cases that developed were kept in line with other typical outbreaks, which "shows that doing good contact investigations is important and preventative therapy works".
In parallel and as shown in Fig. 1, panel B, alcohol-fed mouse lungs showed a decrease of Nrf2 protein expression by approximately 67% (p < 0.05) as compared to control-fed mouse lungs.
Previous work by Chang and coworkers, using a compendium of about 200 publicly available transcriptomic profiles of mouse lungs, showed differences in pathogenicity among respiratory viruses were explained in part by the changes in magnitude of gene expression [ 37].
Likewise, in vivo viral growth in mouse lungs showed increased growth for D125G and the double-mutant 1 day post-infection, with 20- and 16-fold increases relative to wt, respectively.
Nevertheless, CXCR4+/− mice lung showed less metastases compared to wild-type (Fig. 3).
Studies in mouse lung show that pharmacologic disruption of TrxR1 activity also induces Nrf2 [ 42, 43].
HE stain of mouse lung tissues showed a significant disruption of the alveolar units and infiltration of inflammatory cells in the lungs induced by bleomycin, compared with saline injection.
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