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(d) Quantification of lectin staining for mouse lung sections with ImageJ.
To investigate whether migratory neutrophils form NETs in LPS-induced ALI, we first performed immunofluorescence staining of paraffin-embedded mouse lung sections.
Microscopic histopathology of representative mouse lung sections after 12 h of mechanical ventilation (H&E staining, ×10 magnification).
To further assess the lung granuloma defect in Pycard−/− mice, we stained mouse lung sections with Ziehl Neelsen stain to identify Mtb.
Mouse lung sections were made as described above.
BrdU-positive ECs were counted on 25 alveolar profiles in controls and siRNAITSN-treated mouse lung sections.
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(H) Representative immunohistochemistry images of metastasis cells in mouse lung section.
The positive cells in each high-power field (HPF) of mouse lung section were dramatically increased after injection of cationic liposomes, PEI and chitosan.
The representative H&E stained and IHC images of mouse lung tumor sections are shown.
Immunohistochemistry of human and mouse lung tissue sections was performed as previously described [ 14].
We have previously reported that chronic As exposure at similar doses did not induce any histologic changes or overt cell population changes in mouse lung tissue sections (Andrew et al. 2007).
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