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(H) Representative immunohistochemistry images of metastasis cells in mouse lung section.
The positive cells in each high-power field (HPF) of mouse lung section were dramatically increased after injection of cationic liposomes, PEI and chitosan.
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Microscopic histopathology of representative mouse lung sections after 12 h of mechanical ventilation (H&E staining, ×10 magnification).
To further assess the lung granuloma defect in Pycard−/− mice, we stained mouse lung sections with Ziehl Neelsen stain to identify Mtb.
Mouse lung sections were made as described above.
BrdU-positive ECs were counted on 25 alveolar profiles in controls and siRNAITSN-treated mouse lung sections.
Mouse lung sections exposed to control non-immune serum showed faint brown diaminobenzidine precipitate throughout the tissue section, consistent with non-specific background staining.
H&E staining of mouse lung sections indicated that the alveolar destruction caused by KDITSN progresses up to 10d of the siRNAITSN treatment.
In brief, primary anti-Ntrk2 antibody (Abcam, ab33655) diluted 1 200 in the blocking buffer was applied to 10 um thick mouse lung sections and incubated for 30 min.
For analysis of macrophage infiltration, formalin-fixed, paraffin-embedded mouse lung sections (4 μm) were deparaffinized and underwent antigen unmasking with an appropriate buffer.
Paraffin-embedded mouse lung sections were deparaffinized and hydrated in distilled water, stained in hematoxilyn and then subjected to Picrosirius Red (Polysciences) staining as instructed by the manufacturer.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com