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Using a systems approach, we present a temporal analysis of transcriptomic and proteomic profiles measured from mouse lung samples collected during the acute phase of infection in order to better understand both dose-dependent and temporal mechanisms of host responses to influenza infection.
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Confluent MDCK cells (grown in 75 cm2 Roux flasks) were infected with 100 µl of mouse lung sample.
At day 3 and 14-post infection, 44 and 742 genes, respectively, were significantly differentially expressed between vaccinated and unvaccinated mice in lung samples, respectively.
Another limitation was the inability to detect GSNO and SNOs in mouse lung or BALF samples.
One BALB/c mouse had the lung sample test inconclusive and one C57BL/6 mouse had a positive lung sample for MPXV DNA.
For our analysis, we constructed 6 libraries of genomic DNA samples from mouse lung tissues obtained from both wild-type and transgenic mice (for each group, n = 3 biological replicates).
However, viral titers were significantly lower in lung samples from mice on the delayed multiple-dose regimen (D1 multiple) than in those from mice receiving vehicle (P < 0.005) (Fig. 6).
Lung samples from mice infected with WT or ΔPT were analyzed for the expression of IL-17 to determine if there was a difference in the induction of this inflammatory cytokine in response to the two strains.
We observed analogous kinetic effects using lung samples from mice infected with A/California/04/2009 (H1N1), and we show that these effects correlate with morbidity and viral titer.
By analyzing a collection of lung samples from mice infected by A/Vietnam/1203/2004 (H5N1; VN1203), we characterized a signature of transcripts and proteins associated with the kinetics of the host response.
Regarding the comment "lung samples from female mice (and estrogen-treated male mice)", to clarify the dose of estrogen directly within the figure legend, we have modified the text to add the details also presented in the Methods section.
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