Using a systems approach, we present a temporal analysis of transcriptomic and proteomic profiles measured from mouse lung samples collected during the acute phase of infection in order to better understand both dose-dependent and temporal mechanisms of host responses to influenza infection.
One BALB/c mouse had the lung sample test inconclusive and one C57BL/6 mouse had a positive lung sample for MPXV DNA.
At day 3 and 14-post infection, 44 and 742 genes, respectively, were significantly differentially expressed between vaccinated and unvaccinated mice in lung samples, respectively.
Another limitation was the inability to detect GSNO and SNOs in mouse lung or BALF samples.
For our analysis, we constructed 6 libraries of genomic DNA samples from mouse lung tissues obtained from both wild-type and transgenic mice (for each group, n = 3 biological replicates).
In Figure 1 below, we show cryoslices of lung sample obtained from mice fed a (control) maltodextrin diet and chronic (6 weeks) of 20% w/v Meadows-Cook diet.
Standard stereological sample preparation, sectioning, and imaging of mouse lung tissues were performed for semi-automated acquisition of mean linear intercept (Lm) data.
In the present study, serologic tests showed that all mouse serum samples were antihantavirus antibody-negative, and hantaviral antigens were not identified in the mouse lung tissues and HTNV-specific sequences were not detected in human serum samples.
Mouse lung, brain, kidney, liver, spleen, placenta and fetus were immediately fixed in 10% formalin after sampling and embedded in paraffin.
In the mouse lung, ILC2s are defined as Lin−CD90+ICOS+CD25+ST2+CD127+.
