A method is described for isolating Clara cells from the mouse lung that does not require the technique of elutriation.
In addition, β1 integrin engagement by specific antibodies has been shown to enhance histone H3 acetylation in the mouse lung endothelial cell genome through a mechanism that requires PARP-1 [31].
The pH optimum for the mouse lung LPCAT was between 7.4 and 10, and the reaction did not require Ca2+.
E-Cad/Lgr6+ but not c-Kit+, single-cell injections produce lung epithelium in the kidney, and they did not require an extra-stromal compartment like the reported Integrin-α6/β4 mouse lung cells (Chapman et al, 2011).
In the mouse lung, ILC2s are defined as Lin−CD90+ICOS+CD25+ST2+CD127+.
An adult mouse lung consists of about 2.3 Mio.
In the optically cleared mouse lung, we observed branching major blood vessels in transplanted mouse lung (Fig. S7A).
Mouse lung was monitored by small animal micro-CT before transplantation to verify lung damage.
In control experiment, we found that the transplanted SOX9+ progenitors cannot incorporate into non-injured healthy mouse lung or porcine pancreatic elastase-injured mouse lung (data not shown).
Then the mice were sacrificed, and the mRNA levels of indicated genes in mouse lung were detected by RT-PCR.
