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An adult mouse lung consists of about 2.3 Mio.
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The negative control consisted of performing the whole procedure with non-infected mouse lung.
In the mouse lung, ILC2s are defined as Lin−CD90+ICOS+CD25+ST2+CD127+.
In the optically cleared mouse lung, we observed branching major blood vessels in transplanted mouse lung (Fig. S7A).
Mouse lung was monitored by small animal micro-CT before transplantation to verify lung damage.
In control experiment, we found that the transplanted SOX9+ progenitors cannot incorporate into non-injured healthy mouse lung or porcine pancreatic elastase-injured mouse lung (data not shown).
Then the mice were sacrificed, and the mRNA levels of indicated genes in mouse lung were detected by RT-PCR.
In contrast to mouse lung development, our knowledge about WNT signaling in human lung development remains sparse.
Clonally derived SOX9+ BCs were carefully characterized and then transplanted into injured mouse lung.
However, we hardly observed human SPC+ AEC2 in transplanted mouse lung.
(H) Representative immunohistochemistry images of metastasis cells in mouse lung section.
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