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In mouse liver, we demonstrate that the GCN2/eIF2α/ATF4 pathway is essential for induction of the TRB3 gene transcription in response to a leucine-deficient diet.
In mouse liver, we demonstrate that the GCN2/eIF2α/ATF4 pathway is essential for the induction of the TRB3 gene transcription in response to a leucine-deficient diet.
After confirming that MET occurs in the developing mouse liver, we attempted to uncover the relationship between MET and hepatic stem cells, which are related to liver development.
In mouse liver, we found HNF4A1 transcripts to be more common in mouse than in humans or rats (present at 63% in mice v/s 37% in human and 42% in rat; p = 0.05) (figure 4c).
To see whether DICER1 overexpression can clear L. donovani from infected mouse liver, we administered NHA-DICER1-expressing plasmids.
In mouse liver we observed, in addition to the increase in signal, a localization of NanoOrange staining into prominent foci.
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To obtain a list of high confidence DEmiRs in Mir122a -/- mouse livers, we subjected the same pair of wild-type (WT) and Mir122a -/- mouse liver samples to small RNA sequencing and OpenArray assay for miRNA profiling.
In mouse livers, we discovered founder-of-origin effects for a subset of local eQTL that drive differential expression of target genes in a subspecies-of-origin specific manner, suggesting a possible role for these loci in transcriptomic and phenotypic differences between strains.
Having established that liver stage infection can be accurately and conveniently measured in vitro and in vivo by assessing the luminescence of PbGFP-Luccon-infected cells or mice livers, we decided to investigate the suitability of this method for the evaluation of anti-plasmodial drugs.
In this study, we identified Nocturnin as one of the target mRNAs of miR-122 in mouse liver and we propose that miR-122 is important for shaping the appropriate circadian expression profile of Nocturnin.
Given that the proteomic identification of the three substrate candidates was from the cytoplasmic fractions of mouse liver tissues, we further performed the experimental validations with cytoplasmic fractions of 293T cells.
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