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Here, we introduced the glyoxylate shunt into mouse liver to investigate mammalian fatty acid metabolism.
Serum was incubated at RT for 40 minutes with crushed lyophilised mouse liver to capture any non-specific antibodies.
On the other hand, Talasz [19] used purified H1 subtypes from mouse liver to measure their binding to mononucleosomes, classifying them as high (H1.2, H1.3 and H1.4), intermediate (H1.1) and low (H1.5) binding affinity.
use conditional knockout of Cdk1 in mouse liver to explore how cell size impacts transcription and metabolism.
Liu has recently shown that MYC is involved in the carcinogenic response of mouse liver to arsenic, another toxic metal [ 39].
In this study, we have generated conditional knockout Ppp2cα by disrupting the Ppp2cα gene in mouse liver to explore the effects of PP2A on hepatic control of glucose homeostasis and lipid metabolism.
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We next performed ChIP assays in mouse livers to verify PPARα binding to the m Igfbp-2 gene promoter at the chromatin level.
We have shown in this study that L. donovani infection downregulates miR-122 and genes involved in cholesterol biosynthesis in infected mouse livers to reduce serum cholesterol.
We next isolated individual hepatocytes, non-parenchymal cells, and hepatic stellate cells from intact mouse livers to verify the cellular source of miR-214-5p miR-214-5p miR-214-5p
To our knowledge, this is the first report of the use of this lentivirus expression system containing a GFP report gene and U6 small nuclear RNA promoter to deliver shRNA into mouse livers to silence HBV genes.
To further elucidate how CREB specifically regulates subsets of target genes, we performed an integrative computational analysis of the CREB cistrome and fasting-dependent transcriptome in mouse livers to identify genomic features correlated with CREB sites at fasting-responsive genes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com