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To identify this mechanism, we stained mouse liver sections to detect markers of various cell populations.
We validated these model findings with additional experiments on mouse liver sections.
All paraffin-embedded mouse liver sections were scanned with a digital slide scanner (Hamamatsu, Nanozoomer 2.0-RS) and files were analysed with the NDP viewer software.
For immunolocalization in liver tissues, paraffin-embedded mouse liver sections (5 µm) were dried for 1 h at 58 °C, followed by antigen retrieval and incubation with primary antibody (anti-cleaved caspase-3, Cell Signaling, #9661; anti-F4/80, eBioscience, #12 4801 80) in a Ventana automated instrument (Ventana Medical Systems, USA).
(C) Photomicrographs of representative H&E staining of mouse liver sections 6 h post-ConA injection (original magnification, 200× and 400×).
In order to determine its binding activity against native hepcidin we first performed immunohistochemistry on paraffin embedded mouse liver sections.
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To address the regulation of genome-wide transcription, we analyzed mouse liver nascent RNA expression, that is, RNA being transcribed by RNA Polymerase II (Pol II) prior to 3′ end formation (from 12 independent samples in LD, 6 time points per day done twice; see analysis of rhythmic transcription in mouse liver section).
To substantiate the finding of higher insulin receptor phosphorylation in DEP-1 ASO-treated mice, liver sections were subjected to Proximity Ligation Assays (PLA) using co-incubation of antibodies directed against the insulin receptor and phosphotyrosine residues.
(a) H&E staining of mice liver section on day 1 and 7 post PBS and hMSG-EpiPCs injection (White arrows: erythrocyte diapedesis, yellow arrows: diseased hepatocytes).
Furthermore, evaluation of Vdr −/− BDL mouse liver tissue sections indicated altered E-cadherin staining associated with increased bile duct rupture.
In situ hybridization on P20 fresh frozen mouse brain and liver sections was performed as described [15] with a few modifications, omitting the treatment with Proteinase K.
More suggestions(15)
mouse colon sections
mouse testis sections
mouse brain sections
mouse liver tumors
mouse midbrain sections
mouse heart sections
mouse liver hepatocytes
mouse liver tissues
mouse liver MDSCs
mouse limb sections
mouse liver results
mouse embryo sections
mouse atlas sections
mouse lens sections
mouse cryostat sections
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