Approximately 0.5 mg of total RNA per mouse liver was labeled for each array as described in the manufacturer's instructions.
The stability of four control reference genes (GAPDH, TUBB, ACTB, and HPRT) was compared to that of four novel reference genes (vps4a, srp72, mRpL16, and GAK) identified as being highly stable across a set of 197 Affymetrix arrays profiling mouse liver samples from 7 distinct public experiments available in Genevestigator.
Approximately 0.5 mg of total RNA per mouse liver was labeled for each bead array as described by Thornley et al. (2011).
For concreteness, however, this section focuses on oscillations in gene expression due to the circadian cycle, data for which was recorded in a time-series micro-array experiment on mouse liver cells [ 12].
Anti-acetyl-lysine immunoprecipitates prepared from the cytoplasmic extracts of the HDAC6 knockout mouse liver showed more abundant acetylation over a wide array of proteins than the wild-type tissue (Fig. 1B).
Mouse liver RNA was assayed using Murine Genome Mu6500A arrays (Experiment 2).
Mouse liver RNAs were hybridized to Affymetrix MOE430A GeneChips and Sterolgene arrays according to the recommended protocols.
Here, we studied the differential genomic expression of mouse liver cells under adult hyperglycemic and aged normoglycemic conditions using expression arrays.
We used the mouse genomic DNA sequence to test our ability to computationally predict 100 kb regions of the mouse genome that exhibit unusually short (<185 bp) nucleosome array periodicities, compared with the bulk chromatin periodicity of 195±5 bp, in <span class="lh">mouse liver nuclei.
In fact, an extensive array of NRs, eighteen to be exact, are direct targets of BMAL1 in the mouse liver (Table 1) 24.
