The present study has several limitations: First, the use of bidirectionally bred mouse lines possesses inherent conceptual restrictions, limiting the interpretation of results obtained using these animal models.
For this reason, we utilized the cybrid technology to generate mouse embryonic stem cell lines possessing different pathogenic and benign mtDNA mutations (Kirby et al., 2009).
Both cultured and mouse-passaged lines possess strong TATA.
In another study conducted on five cell lines possessing different levels of SULT1A1 activity (mouse L5178Y, no activity; Chinese hamster: V79-Hp-PST, high activity; V79, negligible activity; human: HEK293, higher activity; and Caco-2, low activity), HMF exerted DNA damage when exposed at a concentration of 100 mM for 3 h [42].
We here focused attention on choline metabolism of two HER2-overexpressing EOC cell lines possessing different in vivo growth capability in SCID mice, SKOV3 and its SKOV3.ip cell variant.
To this end, here we report efficiently establishing embryonic stem cell lines possessing in vitro and in vivo pluripotency from NOD-scid Il2rg − / − mice.
Summary of GUS activities in the transgenic lines possessing the AtMYB80 promoter- GUS deletion constructs.
GUS activities in the atmyb80 mutant lines possessing the AtMYB80 promoter- GUS construct.
In the present study using the two human cholangiocarcinoma cell lines, RBE and HuCCT1, we first demonstrated that the CD274low cells derived from the two cell lines possess higher tumorigenecity in NOG mice compared with the CD274high cells.
Moreover, the secondary iPSC lines possessed full developmental potential and produced full-term all-iPSC mice through tetraploid complementation [see Additional file 1: Figure S1A].
