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Disease phenotype analysis in all three mouse lines indicated that most phenotypic parameters (attack rates, incubation periods, lesion profiles and Western blots) were compatible with scrapie phenotypes as were immunohistochemistry (IHC) data from RIII and C57BL/6 mice.
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Study of two independent VGF knockout mouse lines indicates that Vgf gene products regulate fat storage and energy expenditure through a mechanism that is independent of an effect on locomotor activity.
Data obtained from the comparison of different mouse lines indicate that the onset and the severity of the amyloid deposits are directly linked to the level of Aβ42 peptide.
Comparisons of different transgenic mouse lines already indicated that the expression levels of the prion protein were inversely related to the incubation period in classical scrapie [6].
Sporadic CJD transmits to all these mouse lines, as indicated by evidence of clinical signs and vacuolar pathology and/or PrP deposition, and the combination of these transmission properties has resulted in the identification of 4 strains of sCJD (3 ).
Nevertheless, in the current study both sources generated presented fairly high AR and short IP compared to similar inocula that have been previously bioassayed in our institute in all three wild type mouse lines used indicating that both had relatively high infectivity [ 31, 32].
(D ) Viral titers from biopsies of skin or neural tissue obtained on day 6 7 (Control mice) and day 14 (HSV-2 Δ gD −/+gD−1-vaccinated mice) (n = 5 mice per group, lines indicate means).
Viral titers from biopsies of skin obtained on day 7 8 (Control mice) and day 14 (HSV-2 Δ gD −/+gD−1-vaccinated mice) (n = 5 mice per group, lines indicate means).
(G ) HSV-2 pfu in the media of ex vivo reactivated neural tissue obtained from challenged mice (n = 5 mice per group, lines indicate means).
Each symbol represents an individual mouse; small horizontal lines indicate the mean.
Dorsomorphin was also effective at robustly inducing cardiomyogenesis in unmodified CGR8 and R1 mouse ES cell lines, indicating that the procardiogenic effects of dorsomorphin under this condition were not cell line-restricted (Figure 2B).
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