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This vector should therefore be a convenient and versatile tool for the generation of transgenic mouse lines in which a desired protein may be expressed in Ii-positive cells at levels similar to those of endogenous Ii.

The superovulation technique will contribute to the archiving of cryopreserved embryos of genetically engineered mice using small numbers of donors and has the potential to produce more live animals for rederivation of the archived mouse lines in mouse repositories.

In this chapter, we discuss the design considerations and methodologies involved in the production and analysis of transgenic mouse lines in which fluorescent reporters are expressed in the hematopoietic system of the mouse embryo.

Lu's team has already generated 16,000 knockout mouse lines in a systematic attempt to dissect out the function of genes expressed in the brain, and a new research building is already underway (scheduled for completion in 2016) to house this vast resource.

Here we have characterized two independent transgenic mouse lines in which Cre recombinase is expressed under the control of the mouse Plp promoter.

Feng et al. [26] observed various GFP expression patterns among transgenic mouse lines in which GFP expression was driven by the Thy-1.2 gene.

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Our results demonstrate that the DAT KD mouse line in the BPM is a consistent, reproducible model of mania-relevant behaviors.

We employed the Cre recombinase/loxP system to create a mouse line in which PKA activity can be inhibited in any cell-type that expresses Cre recombinase.

Despite the extensive use of the R6/1 mouse line in the last decade, its pre-motor phenotype has not been investigated in detail.

Previously, we generated a conditional mouse line, in which the IFN-β coding sequence is replaced by the reporter luciferase upon cell specific Cre expression [30].

We next examined a BAT-gal transgenic mouse line in which β-galactosidase reporter is expressed under the control of β-catenin/Tcell factor responsive elements [25].

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