Recently we produced and characterized a transgenic mouse model of FUSopathy (FUS-TG mouse line) based on aggregation of C-terminally truncated human FUS (41).
This represents ~2.5% of the cost of production and in vivo phenotyping for a mouse line (based on pathology of four animals and a cost/line of approximately US$50,000), which provides good value for the amount of information potentially gained.
15, 86, 150 Instead of utilizing E-selectin knockout mice, these two studies overexpressed α-1,3 fucosyltransferases (FTs) in E-selectin ligand (ESL -negative human and mousESL -negativenes based on thumanndings thandESL-positive PCa cells highly express FT3, 6 or FT7.
It is possible to select different strains of mice to generate isogenic lines based on the expected effects that a transgene or targeted gene disruption would have on specific behaviours.
We have compiled a database of SNP profiles for hundreds of commonly used mouse inbred strains and cell lines based on the MUGA genotyping array, which is commercially available and affordable (~US$100).
Since Epistem-like B9-2-5 is distinguishable from naïve-like iPSC lines based on the mouse EpiSC-like colony shape and bFGF-dependent proliferation (Fujishiro et al., 2013), Epistem-like B9-2-5 was excluded in the following experiments.
By data mining the DTP archive, we are able to identify compounds that are preferentially toxic against the most tumorigenic of the NCI60 cell lines, based on the take rate of the cell lines in a mouse xenograft model.
The mouse ESC and EB expression data were determined from V6.5 (GSE3231 of GEO database), R1 (GSE2972) and J1 (GSE3749) cell lines, based on Affymetrix chips.
To further examine possible mechanisms that lead to the observed strong phenotype in vivo, we established an immortalized 3T3 cell line based on mouse embryonic fibroblasts (MEFs) of floxed embryos, allowing for the inducible homozygous knockout of Dohh.
