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This variation is greater than between different wild-type mouse lines and suggests that overexpression of PrP causes disturbance to maintenance of isotope ratios at an individual level.
For in vivo imaging, we used mice from three different TG mouse lines and their strain-matched wild-type (WT) control animals.
However, difficulties arise when attempting to critically compare the significance of data obtained in vivo by using different genetically engineered mouse lines and various experimental models.
In conclusion, DiCre represents a valuable new option to establish deleter mouse lines and achieve conditional transgenesis.
We would like to thank Dr. G. Oliver for generously providing technical expertise, mouse lines and suggestions throughout this work.
Chimeric animals were mated to generate heterozygous Cdk2ap1 knockout mouse lines and heterozygosity was verified by multiplex PCR and southern analysis on tail biopsies (Fig. 1).
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Genomic coordinates for the mouse LINE- and SINE-free regions are provided in Supplementary Tables S3 and S4.
The numbers of mouse LINE- and SINE-free regions are thus both around 1.5 higher than in humans.
Twenty lenses were dissected from each mouse line and then immediately subjected to vacuum drying for 48 hours.
We thank C. Manalac and J. Zhong for technical support, Y. Chen-Tsai and the Stanford Transgenic Facility for mouse transgenesis, G. Fishell for providing the Foxg1-tTA mouse line, and S. Sabo for providing the Synaptophysin cDNA.
In the present paper, in order to elucidate the physiological role of tACE3, we produced an Ace3 disrupted mouse line and analyzed the fertilizing ability of these mice both in vitro and in vivo.
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