Sentence examples for mouse lines after from inspiring English sources

We generated mouse lines after successful germline transmission of the knock-in transgene.

Plasma alanine aminotransferase and aspartate aminotransferase activities increased in all mouse lines after exposure to 1,000 and 2,000 ppm TRI.

Genotyping of the FltpZV mouse line (after Cre-mediated excision of the neomycin selection cassette) was performed using the forward primer 566 and 418 as well as the reverse primer 565.

Although the 2A peptide has been shown to function in a wide variety of cell types and in specific tissues in vivo, it has to date not been demonstrated to function in a permanent transgenic mouse line after passage through the germ-line, to test for possible silencing of 2A-containing transgenes.

This specific feature of impaired angiogenesis is also reported for other mouse lines, e.g. after deletion of TGFβ ligands in the entire forebrain (65, 66), deletions of TGFBR2 in EC (26, 41), upon knock-out of ItgaV or Itgb8 in the neuroepithelium (67, 68) and after deletion of Gpr124 (27– 29).

However, four (33%) transmitted transgenic mouse lines (F1) were found after twelve F0 generation animals were randomly selected to mate with wild type mice (Fig. 7).

Several founder mice were obtained and used to establish mouse lines (called in the followings DiCre lines) after excision of the hygromycin-resistance cassette through mating of F1 mice with an Flpe-expressing deleter line.

The transgene construct that harbors the conserved Grhl3 15th intron region linked to a reporter gene, lacZ, was constructed, after which transgenic mouse lines were generated and the β-gal reporter activity of F1 transgenic embryos was analyzed (Fig. 7F G′).

Collagen 12a1 mRNA levels did not change after BLM treatment in both mouse lines (data not shown).

After confirming the functionality and specificity of the construct in vitro, transgenic mouse lines were generated by pronuclear DNA microinjection.

J.S. established all mouse lines.