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To investigate the function of IL-32β in vivo, we generated a transgenic mouse line using the PECAM-1 promoter.
We generated a Tg CAG-flox-dnBmpr1a-NLLacZ 1Nobs mouse line using Tg CAG-flox-dnBmpr1a-NLLacZ 1Nobsa-IRES-NLLacZ (Fig. 1A).
Reporter activity was not detected in the brain or testis of the analyzed rBDNF-lacZ-BAC mouse line using X-gal staining assay.
DOI: http://dx.doi.org/10.7554/eLife.01160.006 To gain insight into Megf8's function during development we generated a conditional knockout mouse line using the Cre/LoxP system.
To examine gp250's effect on the post-selection MCC-specific T cell repertoire, we constructed a transgenic mouse line using the MHC class II promoter in which all of the MHC class II molecules contained the gp250 self-peptide via a covalent linkage of the peptide to the MHC class II β chain.
In the present study, we have generated a transgenic mouse line using a bacterial artificial chromosome (BAC) clone containing 207 kb of rat BDNF (rBDNF) locus, encompassing the genomic region from 13 kb upstream of rBDNF exon I to 144 kb downstream of rBDNF coding exon.
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This could be because of a different mouse line used or a different antibody recognizing another epitope.
We then investigated the expression of myelin proteins in Olig1−/− mice with the PGKneo cassette [12], which are the mouse line used in the present study.
The very restricted expression of ΔGR in the mouse line used in these experiments suggests that the phenotype observed is likely due to the over-activation of the ΔGR in the DG.
The mouse line used was Wnt1cre:R26RYFP (Danielian et al., 1998; Srinivas et al., 2001).
Tg65, the Tg mouse line used in this study, has been maintained on a C57BL/6 genetic background.
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