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In the transgenic mouse line the presence of the luciferase gene and its respective product was tested by different approaches.
Regarding the TgOvPrP4 mouse line, the presence of [TgOvPrP4 (x0.25)] animals which express low levels of PrPc clearly represents a drawback for transmission studies of TSEs.
In the CKO mouse line the Cre-recombinase is expressed in a mosaic fashion in the eye [48] leading to an incomplete deletion of Dicer in the retina with most of the RGCs for example still Dicer-positive [27].
For genotyping of the Dlg3:Venus mouse line, the forward primer 180 and the reverse primer 181 were used.
In N-cadherin conditional mutant mouse line, the first exon containing the start codon is flanked by loxP sites, resulting in a Cdh2 floxed allele (Cdh2fl).
This has been previously published by Konno et al. (2008), and we have here used the same mouse line, the same antibodies and similar protocols.
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In both mouse lines, the proportion of CD62Lhigh T cells strongly decreased during infection.
In this study, we have also used the forebrain-specific αCaMKII driven tTA expressing mouse lines; the tTACaMK (line B) [9] and the tTACN12 [29].
Hence, in these transgenic mouse lines, the nuclear localization of the hCD2 transgene correlates with its expression status.
Rows of animal skulls, from wolves to field mice, line the display cases.
This patchwork epidermal hyperplasia was more severe in the mouse line with the mutant (A339T) transgene than in the mouse line with the wild-type transgene.
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