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We split the Dlk1-Dio3 megacluster into 13 subclusters and stably expressed each in a mouse liver tumor cell line (Supporting Fig. 3A; Supporting Table 3).
To identify cellular sources of IL-33 an Il33 Cit/+ reporter mouse line was generated (Supporting Information Fig. 1A).
This idea is also supported by behavioral results we obtained in another mouse line (Line 23) which expressed the ΔGR in several brain structures but not in the DG (Supplementary Figure S3).
Indeed, this interpretation is supported by a recent study demonstrating that a mouse line carrying a homozygous mouse mutation homologous of V790M (musk v789M/V789M mice) presents no abnormal phenotype, whereas a hemizygous mouse line carrying the V790M allele paired with a null allele (musk v789M /– mice) develops severe weakness (32).
This conclusion is also supported by results from a second membralin KO mouse line we generated using a gene trapping strategy.
We termed this mouse line IL-1R1-restore (IL-1R1r).
This mouse line was designated as ΔCS.
We thank C. Manalac and J. Zhong for technical support, Y. Chen-Tsai and the Stanford Transgenic Facility for mouse transgenesis, G. Fishell for providing the Foxg1-tTA mouse line, and S. Sabo for providing the Synaptophysin cDNA.
UK-2 was indistinguishable from BSE when isolated in SJL/OlaHsd mice; no LP was available for UK-1 as it did not transmit to this mouse line (Supporting Information Figure S3).
All knockout mouse lines were generated and maintained at the University of Nevada Genetic Engineering Center UNGECC) supported, in part, by a NIH COBRE grant (1P30GM110767).
See online Supporting Information for further details on mouse lines.
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