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In this paper, we report the generation of a mouse line displaying an inactivated ATX gene product.
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This mouse line displays distinct changes in the structure of the epidermis and a compromised barrier function, but the phenotype was not nearly as severe as that seen in mutant neonates.
Only in the CA3 region of the hippocampus did the mouse lines display no differences.
These two independent Tg mouse lines displayed similar levels of neurobehavioral abnormalities; hyper-locomotor activity in a novel environment, learning deficits in context-fear conditioning, reduced social interactions, and a nonsignificant trend toward decreasing prepulse inhibition.
None of the cerebellar mouse lines displayed deficits in motivation.
None of the cerebellar mouse lines displayed deficits in avoidance behavior.
Consistent with Groszer et al. (2008), homozygous pups of R552H and S321X mouse lines displayed developmental delays and severe motor impairments, surviving only 3 4 weeks after birth.
After obtaining multiple founder mice, lines displaying comparable levels of mutant or wild-type transgene accumulation were selected and backcrossed to C57Bl6 to establish the lines detailed in this paper.
A second ENU-derived mouse line that displays an HPE-like phenotype was isolated from an independent screen.
In conclusion, we have generated a novel Wcre transgenic mouse line that displays Cre recombinase activity in mammary stem and/or progenitor cells.
Dependent on the background strain, homozygous mutant embryos from the beaker mouse line also display a range of phenotypes – normal appearance in 129S1, anterior truncations in C57 (Fig. 2B,D), and holoprosencephaly (Fig. 2A,C) and/or eye defects (supplementary material Fig. S1C,D) in mixed 129S1/C57 strains.
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