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Taken together, we conclude that the Tubb3-mGFP transgenic mouse line allows the identification of newborn neurons by their intrinsic mGFP fluorescence at the systemic, tissue and cellular level in unfixed and fixed tissue.
This mouse line allows specific distinct between reporter and endogenous expression as hAP is heat-resistant while endogenous alkaline phosphatase is heat-labile.
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The new mouse line allowed to study the requirements for positive selection of human CD1b-restricted T cells and to explore the capacity of these transgenic T cells to protect mice from M. tuberculosis infection.
Use of this mouse line allowed the preservation of Wls expression in the dorsal neural tube and thus its capacity to secret Wnt, despite conditional deletion of Wls in NCCs.
In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner.
To determine whether the Neurog2-d4Venus and Gadd45g-d4Venus mouse lines allow appropriate visualization of cells differentiating into the neuronal lineage, we first examined developing brain specimens immunohistochemically.
In addition, the large data set of expression profiles from 33 mouse lines allowed us to validate known and to newly identify so far unknown genes that are expressed specifically in Treg but not Th cells.
In my previous research, I engineered a mouse line that allows for the indelible labeling of a memory trace.
This study develops the FOS tetracycline transactivator protein (tTa) transgenic mouse line, which allows long-term tagging of neurons that are active during an experience and sets up the ability to manipulate these neurons in the future.
In summary, we describe the first transgenic sponge mouse line that allows for conditional inactivation of an miRNA family in a spatio-temporal-controlled manner.
We addressed these mechanistically important questions using a mouse line that allows inducible Schwann cell-specific Frabin/Fgd4 deletion in adult animals.
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