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Similar results are demonstrated in a case study of the adult mouse limb presented in Section 3.2.
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We have determined that tendon is first clearly present in mouse limb at embryonic day 14.5 (E14.5) and, by in situ hybridization, that scleraxis is expressed in the mouse tendons at E14.5.
The Meis proteins may interact with paired box proteins (PBX), a second class of the TALE family, although evidence has been presented that this interaction is not required for PD specification in mouse limb development (Mercader et al., 2009).
Expression pattern of dkk-1 during mouse limb development.
(H) Sox9 expression pattern in mouse limb buds at E13.5; adapted from Fig. 8M in104.
Therefore, Shh specifically becomes necessary for normal limb development at or just distal to the stylopod/zeugopod junction (elbow/knee joints) during mouse limb development.
(A) The increase in the length of the proximal-distal mouse limb bud axis with developmental time (in embryonic days) measured from forty limb buds images.
Here, we analyze the emergence of different MSC types during mouse limb and long bone development.
Finally, unlike the wild-type mouse limb bud, the Shh mutant posterior limb bud mesoderm does not cause digit duplications when grafted to the anterior border of chick limb buds, and therefore lacks polarizing activity.
Bénazet, J.-D. et al. A self-regulatory system of interlinked signaling feedback loops controls mouse limb patterning.
Ahn, S. & Joyner, A. L. Dynamic changes in the response of cells to positive hedgehog signaling during mouse limb patterning.
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