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Finally, cytotoxicity tests were performed using mouse leukemic monocyte macrophage cells (Raw 264.7) for culture periods of 1, 3 and 5 days, respectively.
To investigate whether this point mutation changes IRF-5 subcellular localization, wild type and point-mutated form of IRF-5 were linked to GFP, stably tansfected into HEK293 cells and RAW264.7 cells (mouse leukemic monocyte macrophage cell line), and examined for leptomycin B (LMB -induced changes in subceLMB -inducedization.
The mouse leukemic monocyte macrophage cell line, RAW 264.7, was maintained in DMEM.
Mouse leukemic monocyte macrophage RAW 264.7 cells are derived from Abelson-virus-treated mice ascites and recognized as pre-OCs [ 24].
RAW 264.7 (Mouse leukemic monocyte macrophage cell line) were obtained from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China).
RAW 264.7, a mouse leukemic monocyte macrophage cell line, was grown at 37°C in a 5% CO2 atmosphere in DMEM (Invitrogen, USA) containing 10% fetal bovine serum (FBS).
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The majority of their curated MS data has been generated using mouse leukemic monocyte-macrophage cells and, while extensive, does not yet represent all biological lipids.
The cytotoxicity investigation on mouse melanocyte B16-F100) and human leukemic monocyte lymphoma (U937) cells was done using sodium 3'-[1- phenyl amino-carbonyl)-3'-[1- phenylum]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene amino-carbonyl -3,4-tetrazolium]-bis-[4-methoxy-6-nitrobenzene
CD11c+ cells isolated from mouse lung, BMDCs, monocyte-derived (MD DCs or RAW 264.7 cells (mouse leukemic monocyte/macrophage cell line) (ATCC, Manassas, VA) were treated with indicated amount of nCB for 1 or 2 days, were washed and placed in co-culture assays with or without T cells (at 1 10 ratio).
Although BC-1258 increased FBXL2 protein levels, it decreased FBXL2 substrates, including Aurora B in human leukemia cells (U937 (human leukemic monocyte lymphoma cell line), K562 (human erythroleukemic cell line) and THP1 (human acute monocytic leukemia cell line)).
Among them, the most potent compound 8 had the IC50 values of 0.56 μM for HL60 (Human promyelocytic leukemia cells) tumor cell line and 0.58 μM for U937 (Human leukemic monocyte lymphoma cells) tumor cell line.
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