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Grx1 knockout mice show increased cellular protein S-glutathionylation42, and Grx2 knockout increases sensitivity to oxidative stress in mouse lens epithelial cells43.
Wu, H., Lin, L., Giblin, F., Ho, Y. S. & Lou, M. F. Glutaredoxin 2 knockout increases sensitivity to oxidative stress in mouse lens epithelial cells.
We use mouse lens epithelial cells to demonstrate that cells can populate the internal surfaces of the chambers within a week to create numerous hollow spheroids.
Mouse lens epithelial cells (αTN4-1) were grown to 95% confluence.
Similar expression patterns of the above factors were observed in the mouse lens epithelial cells, αTN4-1 (data not shown).
We have now analyzed in depth the alterations caused by the expression of Dbl oncogene in the mouse lens epithelial cells.
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Mouse αTN4-1 lepitheliallial cells were cultured as described elsewhere (Yang and Cvekl 2005).
Foxe3 continues to be expressed in the mouse lens epithelium until adulthood, being indispensable for a complete closure of the lens vesicle and for the maintenance, proliferation and survival of the lens epithelial cells [ 37].
In addition to its essential role in the optical and refractive properties of the eye lens, αA-crystallin performs other functions; αA-crystallin knockout mice exhibit increased lens epithelial cell death and reduced cell proliferation [ 26, 27], and αA-crystallin is also expressed in the retina, brain, spleen, and thymus [ 28] although its role in these tissues is not fully understood.
These findings suggest that ALR2 over-expression is associated with an alteration in the balance between proliferation and apoptosis of epithelial cells in the mouse lens, and that cells associated with epithelial plaques in the PAR39 lens have features in common with cells undergoing EMT.
Global induction of apoptosis with etoposide, cycloheximide or puromycin was shown to lead to a loss of cell coupling, probably due to caspase-3-mediated degradation of Cx43, in primary bovine lens epithelial and mouse NIH3T3 fibroblasts [ 38].
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