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Before investigating the possible differences of gene expression in the temporal cortex of young adult, elderly and "AD-like" grey mouse lemurs, we compared the anatomical shape of the brains from the three groups (Fig. 1).
By comparing responses of different tissues from aroused versus torpid gray mouse lemurs, we explore the regulatory control of transcription and translation during torpor.
In mouse lemurs we found a right ear-left hemispheric advantage toward conspecific communication sounds of negative valence for males, but not for females or the whole population.
However, in mouse lemurs we did not find a relation between individual hand preference and ear-hemispheric advantages toward conspecific communication sounds.
To examine the response of the energy-sensing AMPK signaling pathway to daily torpor in gray mouse lemurs, we measured the relative level of p-AMPKα (Thr172) in torpid and aroused lemurs.
Because it is difficult to collect a high number or high volume of blood from the Grey Mouse Lemurs, we collected 200 μL of total blood from each female and used two distinct VetScan Comprehensive Diagnostic Profiles per sample.
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For each mouse lemur species, we fitted negative binomial distributions of detection events combined with a natural log-link function to logarithmic encounter rates (overdispersion indicated that Poisson models were prone to Type I errors).
Using standard consensus sequences of Alu repeats [ 13] to screen all available genomic sources of primates, represented by human and macaque (both Catarrhini), marmoset (Platyrrhini), Philippine Tarsius (Tarsiiformes), and gray mouse lemur (Strepsirrhini), we extracted and analyzed more than 9,300 nested from 4.5 million detected Alu SINEs.
We sampled mouse lemurs at six sites.
We trapped mouse lemurs within the gallery and the surrounding spiny bush.
At Ambatoabo (Abt), we trapped mouse lemurs in a dense gallery forest and in adjacent dry spiny bush.
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