Sentence examples for mouse labeled with from inspiring English sources

Cells were rinsed three times in PBS buffer and incubated overnight at RT with the secondary antibodies (goat anti mouse labeled with Alexa fluor 546, Invitrogen Corp. Carlsbad CA, USA) diluted to 1/50 in 1% BSA containing buffer.

Database information provides independent confirmation of several of the untranslated exons identified in this study (-1, -2a, -2b and -2c in the mouse; and -1, -2a in human), as well as additional -2 exons in the mouse (labeled with an arrowhead in Figure 4B) which we have thus far not found by 5'RACE both in mouse and human (>50 independent 5'RACE products sequenced; detailed data not shown).

Figure 1A shows a typical Purkinje cell in the 10-day-old (P10) mouse labeled with enhanced green fluorescent protein (EGFP) by AAV-mediated gene transfer (Hirai, 2008; Kaneko et al., 2011).

Membranes were incubated for detection with secondary antibodies raised against rabbit labeled with CyDye800 (Licor, Bad Homburg, Germany) and mouse labeled with CyDye700 (Licor, Bad Homburg, Germany) for 1 h at room temperature.

EnVision uses a secondary antibody against both rabbit and mouse that is directly labelled with HRP (horseradish peroxidase) reacting with DAB, whereas LSAB uses a secondary antibody against rabbit and mouse, labelled with biotin, then streptavidin-HRP is added and the staining is done with DAB.

As internal control, 107 splenocytes from normal B6 mice labeled with low concentration of CFSE were co-injected (Fig. 4A, middle peak).

B cells from BALB/c mice labeled with 25 µM 7-amino-4-chloromethylcoumarin (CMAC, blue; Molecular Probes™ / Invitrogen™) for 45 min at 37°C; 24 hr later these mice were s.c. injected with 100 nmol CFSE (green) and 20 pmol QD (red) included in 50 µl CFA.

The membrane was washed with TBST, and then incubated in 1 20 000 diluted anti-mouse labeled with horseradish peroxidase (HRP) (GE Healthcare) for 1 hour.

Afterwards the cover slips were washed in PBS three times and then incubated with a secondary goat anti-mouse labeled with Alexa 488 fluorescent antibody or goat anti-rabbit labeled with Alexa 594 fluorescent antibody (Molecular Probes, Karlsruhe, Germany).

Subsequently, specimens were rinsed in 0.1 % PBT three to six times and incubated with the appropriate secondary antibodies conjugated with fluorochromes (also mixed 1 1 in a final concentration of 1 200; goat anti-mouse labeled with CY5 (JACKSON IMMUNO-RESEARCH, West Grove, USA, 115-175-062), goanti-rabbitbit labeled with TRITC (SIGMA T5268)) for up to 24 h at RT.

To compare the fluorescence with that of YFP, we imaged nerve terminals in triangularis sterni (TS) muscles dissected from thy1.2YFP16 mice and non-YFP mice, labeled with GFP-HcBoNTx/A.