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Mice kidneys: Proteins were extracted from frozen mouse kidneys using RIPA [43] For microscopy, kidneys were fixed in 4% formaldehyde.
Immunohistochemical staining of sections of 14.5dpc embryonic mouse kidneys using an antibody against Esrrg showed expression in the future collecting ducts and kidney capsule.
Wholemount in-situ hybridization (WISH) was performed on 14.5dpc embryonic mouse kidneys using riboprobes designed to detect expression of the mouse orthologs of the genes at the t(1 2) breakpoints.
Further, it was studied in adult and fetal mouse kidneys using reverse-transcription polymerase chain reaction.
RNA was extracted from whole mouse kidneys using acid guanidinium thiocyanate-phenol-chloroform extraction [ 20].
Results In this study, PCKSs were prepared from healthy C57BL/6 mouse kidneys using a Krumdieck tissue slicer.
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Microbubble circulation persistence was measured in the healthy mouse kidney using a Visualsonics Vevo 770 scanner operating at 40 MHz in fundamental mode.
We previously generated an atlas of gene expression in the developing mouse kidney using microarray analysis of anatomical compartments collected via laser capture microdissection.
The engrafted human E-Cad+/Lgr6+ cells were distinguishable from the mouse kidney using a commercial specific human nuclear antibody.
Sun et al. [ 49] identified five miRNAs (-192, -194, -204, -215, and -216) that were highly expressed in human and mouse kidney using miRNA microarray.
Mouse tissue total RNA was prepared from whole kidneys using Trizol® (Invitrogen).
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