Mouse kidney, testis, epididymis and vas deferens were fixed for 6 h in Bouin solution, dehydrated and embedded in paraffin.
Mouse kidney, testis, epididymis and vas deferens were isolated and minced in homogenization buffer (100 mM NaCl, 10 mM HEPES, 2 mM EDTA, 1 mM DTT, 2% Triton X-100) on ice.
Immunoprecipitates of endogenous SPAK or OSR1 derived from mouse kidney or testis were analysed by mass spectroscopy.
In response to anoxia, an increase in VEGF concentration and an altered pattern in the expression of VEGF receptor have been found in hippocampal neurons in vitro and in mouse brain, kidney, testis, lung, heart, and liver in vivo [24] [26].
LARGE2 has been cloned from human kidney, mouse kidney and mouse testis RNA, and the two paralogs have different tissue expression profiles: LARGE is widely expressed, with levels highest in the brain, heart and skeletal muscle; and LARGE2 is highly expressed in the kidney and the placenta, but not in the brain (Fujimura et al. 2005; Grewal et al. 2005).
Northern analysis showed that Dlk1 is expressed in many adult mouse tissues, including brain, kidney, testis, and thymus.
We extracted expression data for 5 organs in mouse (cerebral cortex, liver, kidney, testis and heart) and human (brain, liver, kidney, testis and heart).
In line with observations from Ball et al. [4], [5], the highest levels of IDO2 mRNA were observed in the mouse liver, followed by the kidney, testis and brain tissues.
Eleven mouse cell lines, representing liver, kidney, testis, mammary gland, embryo, alveolar macrophages, skin, muscle, macrophage, T-lymphocyte and B-lymphocyte, were chosen for UMRR [ 21].
When tissue-specific differentially methylated regions (T-DMRs) in human and mouse tissues including heart, colon, kidney, testis, spleen, and muscle were compared, they could be distinguished clearly according to the corresponding tissues based on their methylation status [ 27].
A 40-cycle semi-quantitative PCR reaction failed to detect otolin transcripts from seventeen major adult mouse tissues (heart, brain, spleen, lung, liver, muscle, kidney, testis, placenta, eye, lymph node, smooth muscle, prostate, thymus, stomach, uterus, adipose tissue).
