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A smaller area of an adjacent mouse kidney section was imaged with 10 μm pixel size in order to investigate the distribution of imatinib in more detail.
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Autoradiography was performed to qualitatively measure relative 64Cu intensity in mouse kidney sections.
Notably, we did not observe any thrombi in the Ehd3 /–; Ehd4–/– mouse kidney sections analyzed despite other hallmarks of TMA.
To establish βarr2 targeting to PC in vivo, distribution of βarr2 was analyzed in mouse kidney sections (Figure 4F), where PCs are located at the luminal side (Lu) of tubular epithelial cells [22].
PAS stained Ehd3 /–; Ehd4–/– mouse kidney sections showed characteristic glomerulomegaly, thickening and duplication of the GBM, expanded and lytic-appearing mesangium, variable degrees of mesangial interposition and abnormal capillary loops (Figure 5A B).
No expression could be seen in the adult mouse kidney sections examined (data not shown).
Confocal microscopy demonstrated extensive and dense cytoplasmic staining for VLPs with anti-VP1 antibody in mice kidney sections, following tail-vein injections (Figure 3).
Some mouse kidney paraffin sections were stained with hematoxylin and eosin (H&E) by routine methods.
The presence of neutrophils as seen in the H & E sections was confirmed by staining the mouse kidney and liver paraffin-wax sections for the presence of chloroacetate esterase; the neutrophils stained red and the sections were counterstained with Mayer's haematoxylin.
The axial resolution improvement is further demonstrated with a prepared slide of sectioned mouse kidney (F24630, Invitrogen, Carlsbad, CA).
Sectioned mouse kidney tissue samples were mounted on ITO coated slides and desiccated at room temperature for 20 min. An ImagePrep spray station (Bruker Daltonics) was used to coat the slide with a 0.2 mL solution containing 8 mU of the bCDase or 2 mU of the bSMase enzyme.
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