The presence of neutrophils as seen in the H & E sections was confirmed by staining the mouse kidney and liver paraffin-wax sections for the presence of chloroacetate esterase; the neutrophils stained red and the sections were counterstained with Mayer's haematoxylin.
Wild-type and GPRC6A mouse kidney were routinely processed and embedded in paraffin.
An overview showing intensity distributions for exemplary m/ z-values together with the mean spectrum for each dataset is provided in Additional file 2. The dataset comprises 75 sections from the central part of a mouse kidney that was PAXgene® fixed and paraffin embedded.
To analyse the expression of Ki-67, slides of fixed and paraffin-embedded mouse kidneys were de-paraffinized using Xylol and descending concentrations of ethanol.
Adjacent 4-μm formalin-fixed paraffin-embedded human and mouse kidney sections were immunohistochemically stained for LSD1, IL-6, MCP-1, CD68, H3K9me1/2, TLR4 (Abcam, Cambridge, MA, USA), and IL-1β (Cell Signaling Technology, Danvers, MA, USA) protein expression with an avidin-biotin-peroxidase complex method.
Paraffin-embedded sections of mouse kidney tissue and human colon carcinoma were used as positive controls for p-Akt1/2/3 and CIP-2A, respectively, as described in the datasheet from the manufacturer.
Paraffin-embedded sections of mouse kidney tissue and human colon carcinoma were used as positive controls for p-Akt1/2/3 and CIP2A, respectively, as described in the datasheet from the manufacturer.
Mouse kidney samples were fixed in 10% formaldehyde, embedded in paraffin and cut into 4 μm sections.
Human and mouse kidney samples were fixed with formaldehyde and embedded in paraffin.
Right mouse kidney tissue samples were fixed in 4% formalin and processed to paraffin blocks.
