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Following surgery, the cover was placed over the box and the mouse kept with the abdomen open exposed to the insufflated gas.
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The only difference was that instead of a laparoscopy with a pneumoperitoneum, a laparotomy was performed and the mice were kept with the open abdomen in a box exposed to the specific gas environment.
The mice were kept with water containing antibiotics (1.1 g/L of neomycin sulphate) until further analyses.
Adult C57 BL/6 mice were kept with free accesses to food and water.
For testing both predictions, we studied male mice kept either with or without female odors.
We examined 34 weeks old male mice kept in cages with a running wheel for 7 weeks from 19 to 25 weeks.
In standard husbandry of mice, pups are kept with their mother for the first three weeks after birth, during which their nutrition primarily comes from mother's milk.
Despite higher leptin concentration in the HFD fed animals, food intake barely reduced and mice kept accumulating fat, consistently with other studies [ 24].
No significant differences in body weight were found in HU mice compared with age matched control mice during all the experimental periods, nor with mice kept in HU for 14 days plus 14 days after reloading of hind limbs.
In contrast, Pearson et al. showed improved endothelial function in mice kept on a diet with a very high resveratrol content (2400 mg/kg/food) that could be mediated by SIRT1 activation [ 28].
(a,b) Two-month-old WT or Sesn2−/− mice kept on LFD were injected with Tm (500 mg per kg body weight, i.p.).
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