Sentence examples for mouse islets with from inspiring English sources

The self-condensation of human and mouse islets with endothelial cells not only promoted functionalization in culture but also massively improved post-transplant engraftment.

To investigate the effect of chronic high-glucose treatment on expression levels of insulin and ER stress response genes, we treated INS-1 832/13 cells and primary mouse islets with increasing concentrations of glucose for 24 and 72 hr, then measured expression levels of Insulin 1 and Insulin 2 gene expression, as well as expression of several well-known markers of ER stress.

To validate the relevance of synergistic effects of glucose and palmitate on pancreatic β-cells we treated isolated primary mouse islets with 11 mM or 30 mM glucose in either the absence or presence of 400 µM palmitate for 72 hours.

α Cell dysfunction and hyperglucagonemia is induced by postprandial lipemia in healthy subjects and by treating mouse islets with triglyceride-rich lipoproteins (Niederwanger et al., 2014).

Treatment of ND mouse islets with oligomycin, a blocker of the mitochondrial ATP synthase, converted the inhibitory effect of glucose on glucagon secretion into stimulation.

86 In addition, fatty acids can have a toxic effect on β-cells, since treatment of human or mouse islets with palmitate induces apoptosis.

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) showed that the expression level of Msi2 was several fold higher compared with Msi1 in mouse islets, consistent with the results obtained from gene expression databases.

In line with our studies, other reports showed mouse islets treated with TCF7L2 siRNA displayed abnormal glucose-stimulated insulin secretion.

Islet hormone secretion was measured from isolated mouse islets treated with selective GPR120 agonists.

In culture, mouse islets treated with NECA showed an increase in the rate of β-cell proliferation.

Bioluminescence profiles of Per2::luc mouse islets synchronised with 30 min pulse of 100 nmol/l dexamethasone (DEX), 50% horse serum (HS) or 10 μmol/l forskolin (FORS).