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Insulin release from 1.1 B4 human clonal β-cells and mouse islets was significantly increased by zfGIP (10 nM and 1 μM).
Oxygen consumption in single rat or mouse islets was measured at 37°C in the presence or absence of 10 nM Ex-4 or 10 µM forskolin by the self-referencing method based on an electrochemical oxygen sensor (BioCurrents Center, MBL, Woods Hole, MA) moving between a "near" and "far" position at the islet.
Astonishingly, Pax8 mRNA in mouse islets was undetectable while human islets exhibited low levels.
Furthermore, the suppression of GAD in NOD mouse islets was shown to protect the mice from developing diabetes [ 36].
The use of human and mouse islets was approved by the local ethical committees (Dnr 2010/006 for human islets and Dnr C106/11 for mouse islets).
Importantly, a temporary wave of cell cycle gene mRNA expression, measured as a sharp peak of cell cycle gene activity at mid-gestation in mouse islets, was followed by a period of about 1 week of enhanced beta cell proliferation.
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Mouse islets were isolated by collagenase digestion of the pancreas according to previously described procedures (Martinez et al. 2006).
Mouse islets were isolated as described previously [36].
Mouse islets were isolated by bile duct perfusion and collagenase digestion as described before [5].
Mouse islets were isolated and purified by collagenase digestion as described previously [6].
Mouse islets were isolated from pancreatic digests as previously described [59].
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