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We studied the mRNA expression profile of mouse islets during pregnancy to gain a better insight into these changes.
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Acute insulinotropic effects were investigated in isolated mouse islets and during an intravenous glucose tolerance test in normal and diabetic Zucker fatty diabetic (ZDF) rats.
Total RNA extracted from C57Bl6/J mouse islets every 3 days during pregnancy was hybridised on commercially available expression arrays.
Western blot analysis revealed a significant decrease in ZnT8 protein abundance during hypoxia exposure in mouse islets (Fig. 2d).
In summary, human and mouse islets share common architectural features as endocrine micro-organs.
Mouse islets were isolated as described previously [36].
At least five islets were chosen randomly from control or βIRKO mouse islets and ∼4 randomly selected β-cells from each islet were photographed.
Isolated mouse islets.
Consequently, human islets cannot be directly compared with mouse islets.
Mouse islets were acquired through the Cell and Islet Isolation Core Facility at the University of Virginia DERC (DK063609).
Islet hormone secretion was measured from isolated mouse islets treated with selective GPR120 agonists.
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