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Our results, using intact mouse islets, differ from a previous report on a clonal line of β-cells, MIN6 transfected with a cAMP reporter [33].
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These data provide clear evidence that NAD(P H oscillations in islets differ from one mouse to the next, as we have observed in other intracellular processes.
Insulin content did not significantly differ between WT and ZnT8−/− mouse islets in normoxia or hypoxia (ESM Fig. 6).
In summary, human and mouse islets share common architectural features as endocrine micro-organs.
PSN632408 and an endogenous ligand of GPR119, oleoylethanolamide (OEA), can stimulate β-cell replication in mouse islets in vitro.
Mouse islets were isolated by collagenase digestion of the pancreas according to previously described procedures (Martinez et al. 2006).
Mouse islets were isolated as described previously [36].
At least five islets were chosen randomly from control or βIRKO mouse islets and ∼4 randomly selected β-cells from each islet were photographed.
Isolated mouse islets.
Consequently, human islets cannot be directly compared with mouse islets.
Mouse islets were acquired through the Cell and Islet Isolation Core Facility at the University of Virginia DERC (DK063609).
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