They also suggest that although neonatal mouse intestine is structurally fully developed, with the proper crypt-villus organization similar to that in the adult mouse, the embryonic/neonatal mouse intestinal stem cells are molecularly distinct from those in the adult mouse intestine.
Consistent with the absence of Paneth cells, which are essential components of the mouse intestinal stem cell niche (Sato et al, 2011), isolated crypts from Src Fyn Yes KO intestines were unable to form organoids in culture (Sato et al, 2009; Fig 6L P).
More interesting yet, mouse Snai1 is specifically expressed and required for stem cell maintenance in the crypts of the mouse intestine and expands the stem cell population when overexpressed (Horvay et al, 2011; Horvay et al, personal communication).
Unlike the mouse intestine, which contains proliferating stem- and transit-amplifying (TA) cells, only the stem cells are proliferative in the adult Drosophila midgut (Micchelli & Perrimon, 2006; Ohlstein & Spradling, 2006).
In the mouse intestine, a population of equipotent stem cells competes for contact with the Paneth cells, which provide a short-range stem cell competence signal (Sato et al., 2011).
One of the most interesting findings on LGR5 is its specific expression in the stem cells of mouse intestine [17].
ASCL2 is located in stem cells and its knockdown results in the loss of stem cells in the mouse intestine of an in vivo model (Van der Flier et al, 2009).
For normal stem cells, this is readily demonstrated in the mouse intestine by the recruitment of a novel population of stem cells following DNA damage.
To determine the impact of an oncogenic K-ras mutation on crypt fission in adult mouse intestine, we induced sporadic lineage tracing in Lgr5 stem cells containing R26R-Confetti.
In previous reports, Bjerknes et al [16] and Potten [23], [54] estimated that there were 4 6 stem cells in each crypt of mouse intestine (in three dimension).
