Histological changes were evaluated in infected mice on intestinal sections that were H&E stained.
Immunohistochemistry was performed on intestinal sections from the mice kept on a 5-ASA diet as well as the untreated control group.
To assess the extent to which GPBAR1 is responsible for bile acid stimulated GLP-1 release from L-cells, we measured GLP-1 secretion from GPBAR1-knockout mouse intestinal cultures and Ussing chamber-mounted tissue sections.
To identify the transgene-expressing cell population, we stained frozen intestinal sections from lentiviral-infected mice with antibodies to GFP and DsRed.
In order to understand the cell cycle dynamics of adult intestinal stem cells, we analyzed proliferation of CBCs on intestinal sections of Lgr5-GFP mice using double immunofluorescence analysis (Barker et al, 2007; Yan et al, 2012).
To determine if, in our Fas-deficient model, the levels of Fas-L within the adenomas were altered, immunostains of Fas-L were performed on intestinal sections of ApcMin/+ and ApcMin/+/Faslpr mice (Fig. 5).
The co-localization was most pronounced in intestinal sections of Arfrp1 vil−/− mice, but also visible in the controls (see white arrows in higher magnifications; Fig. 6A and B).
As previously suggested, a representative immunofluorescence assay performed on intestinal sections of wild-type C57BL/6J mice demonstrated an intact network of ZO-1 and occludin proteins which were predominantly localised along the apical cellular border.
Remarkably, staining of intestinal sections from TSC2-mutant TG mice for lysozyme, an early marker of Paneth cell differentiation, revealed that almost all the crypts were completely devoid of Paneth cells.
In addition, no differences in the subcellular localization of FATP1, FABP1 and DGAT2 were detected in the intestinal sections of Arfrp1 flox/flox and Arfrp1 vil−/− mice (Supplementary Material, Fig. S4B).
