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Lamina propria pDC in response to mouse intestinal flora promote IgA production.
Pediocin PA-1 did not affect the composition of the mouse intestinal flora [ 62].
The HBF-inoculated tissue metabolite profiles were then compared with those from conventional (that is, gnotobiotic mice transfected with normal mouse intestinal flora) and conventionalized mice (gnotobiotic mice transfected with HBF).
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Two weeks after the end of the WIRS, we took the same parts of faecal samples for bacterial genome extraction and detected the Blab/c mice intestinal flora colonisation using Miseq methods.
In all mice a gradual decrease in the number of P. aeruginosa CFU was measured in lungs without differences between VRE colonized mice, mice with intestinal gram-negative overgrowth due to vancomycin treatment but without VRE colonization, or mice with normal intestinal flora (Fig. 1).
The enhanced sensitivity to low-dose STZ was not recapitulated in WT mice denuded of intestinal flora upon the long-term administration of broad-spectrum antibiotics.
This hypothesis predicts that denuding WT mice of their intestinal flora enhances sensitivity to low-dose STZ treatment and recapitulates the Myd88−/− phenotype.
The most important result of this study was that the response to respiratory tract infection with Pseudomonas was virtually identical, despite major differences in the intestinal flora, for mice colonized and not-colonized with E. faecium.
Differences in intestinal flora (transgenic mice were bred in house) as well as other host factors may play a role in susceptibility to severe disease.
Significant alterations in the composition of the intestinal microbiota have been identified in obese mice, suggesting that differences in intestinal flora may explain some of the risk for obesity [ 129].
Hazen's group further demonstrated that a cocktail of broad-spectrum antibiotics could suppress intestinal flora in mice and prevent production of atherogenic TMAO from the choline in ingested egg yolk lecithin.
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