Sense and antisense PCR primers were designed based on mouse sequence information for the amplification of genes of interest (Table 1).
In conclusion, the results of this study provide comprehensive data about the structure, splicing pattern, and expression of NFAT genes in human and mouse, providing useful information for future studies aiming to elucidate the functions of different NFAT isoforms.
With the exception of those from humans and mice, annotated TSS information for most of the mammalian genomes is not available, and correct TSS information is crucial for the identification of evolutionarily conserved TFBSs based on motif-based alignments.
Because the aGEM Platform is primarily designed to store spatial and temporal gene-expression information for the mouse, the Mouse Genome Informatics Identifiers (MGI) established by the Mouse Genome Informatics Resource have been taken as the standard.
Mouse gene Entrez identifiers were then converted to their human counterparts using homology information for mouse genes collected in the hom.Mm.inp Bioconductor annotation library.
Empirical studies on the ecology of the wood mouse have provided sufficient information for the species to be modelled mechanistically.
We obtained SCL information for mouse proteins from the LOCATE database [ 58], a rigorously expert-curated resource based on independent empirical investigation and additional literature.
Taking advantage of the availability of complete genome sequence information for mouse and human, the most widely used systems for the study of mammalian genetics, we have built a database, [X]uniqMAP, that stores the precalculated unique regions for all transcripts of these two organisms.
An additional 479,270 contigs hit known protein-coding mouse and rat genes, providing CHO-specific sequence information for 17,883 mouse and 19,481 rat genes.
Aylor et al. (2011) used HAPPY (Mott et al. 2000) to generate diplotype probability matrices for all 184 mice based on genotype information for 16,159 markers across the genome.
