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Mouse infection experiments and histopathological and cell analyses.
Mouse infection experiments showed that STB strains are less persistent and virulent than M. tuberculosis.
D.R. and S.C. performed SNP analyses and database management, with the support of G.S. C.R., A.P., W.F., R.S., A.-S.D., E.W., A. Cascioferro and M.-C.G. performed mouse infection experiments, in vivo data analyses and/or mycobacterial growth assays.
The authors thank K. Iyer and L. Carfrae for assistance with mouse infection experiments, and M. Mulvey from the University of Manitoba for providing the environmental mcr-1-positive E. coli isolates.
Mouse infection experiments were performed as described [29] with some modifications.
Supernatant was harvested, spun down to remove cellular debris and used for mouse infection experiments.
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Authors TMT carried out the mouse infection experiment.
The hemolytic L. innocua FSL J1-023 was considered non-pathogenic based on its avirulence in a mouse infection experiment [ 22].
Cultures started from isolated colonies were grown for 2 days in brucella broth at 37°C and used for mice infection experiments.
This pool was split into an aliquot of 100 µl of bacterial suspension that was cultivated on LB agar or LB broth for 48 h at 37°C (control) and another aliquot of 30 µl (7.5×106 cfu) that was used for the intratracheal mice infection (experiment).
Mouse influenza virus infection experiments were carried out under BSL-2+ conditions and were authorised by the Institutional Ethics Committee on Experimental Animals of the VIB Department for Molecular Biomedical Research at Ghent University.
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