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Spleen cells from a mouse immunised with B. ovis faecal antigens were used to produce hybridomas which were screened by ELISA to identify those producing monoclonal antibodies (mAb) to the allergens purified by IgE immunoaffinity chromatography.
One mouse immunised with pcDNA3 also responded with a titer of 65.
In contrast, treatment with a conventional anti-prion antibody derived from mouse immunised with recombinant PrP protein was unable to prevent recurrence of PrPSc replication.
Therefore, the most convincing murine model of RA is that of the recently reported DR4-IE transgenic mouse immunised with citrullinated fibrinogen [ 9].
We therefore wished to determine whether the protein antigen array could be used for the simultaneous screening of hybridoma supernatants from a single mouse immunised with many different zebrafish antigens.
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There was a marked increase in cytokine production in mice immunised with the tPA-containing constructs.
Those vaccinated with BCG lasted 65 days, while mice immunised with Ikeplus survived for 135 days.
When mice immunised with ChAd63/MVA were challenged with live Plasmodium berghei sporozoites, microneedle-mediated ChAd63.ME-TRAP priming demonstrated equivalent protective efficacy as did ID immunisation.
The ability of GC90V to elicit a PTH-rP-specific cytotoxic T cell (CTL) response was demonstrated in BALB/c mice immunised with intranasal (i.n).n
Immunisation with recombinant NcSRS2 iscoms resulted in production of substantial antibody titres against N. caninum antigen, while the mice immunised with M5 iscoms produced only very low levels of antibodies reacting with N. caninum antigen.
Significant GnRH-specific IgG titres were detected in sera obtained from mice immunised with four of the seven lipopeptides tested, with an increase in titres observed after successive immunisations.
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Justyna Jupowicz-Kozak
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